Hi,
Please refer to the following samples on how you can save and restore a Docking layout.
http://www.syncfusion.com/uploads/redirect.aspx?file=SaveAndRestoreLayout_59ef54ad.zip&team=supportSaveAndRestoreLayoutToMemoryStream - will explain how you can save and restore the layout using memory stream.
SaveAndRestoreLayoutToXmlFile - will explain how you can do this with an xml file.
Please try this and let me know if this helps.
Regards,
Nanda