Hi Hockett,
Thank you for your interest in Syncfusion products.
Yes. It is possible to assign multiple Autofilter ranges to the same sheet with XlSIO. Please refer the below code snippet:
[C#]
IRanges rangesOne = sheet.CreateRangesCollection();
rangesOne.Add(sheet.Range["A1:C7"]);
sheet.AutoFilters.FilterRange = rangesOne;
Here is the sample for your reference:
http://websamples.syncfusion.com/samples/XlsIO.Windows/F73791/ExcelAutofilter/main.htmPlease try this and let me know if this helps.
Regards,
G.Yavana