To reset an hierarchical grid, you need to do 2 things.
Me.GridDataBoundGrid1.DataSource = Nothing
Me.GridDataBoundGrid1.Binder.ResetHierarchyLevels()
To use ResetHierarchyLevels, you must have version 1.6.1 as it was not in 1.5.2. The current eval download is 1.6.1.
Here is a sample.